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Starting Oligo
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When you launch Oligo you need to open a sequence file for analysis. Use File-Open menu or double click on the sequence icon. The File-Open window, shown below, lets you choose desired sequence file.
Since Oligo opens several kinds of documents you may set the dialog to "Open As: Nucleic Acid" if it shows something else. Sequences are recognized by Oligo if they have *.seq extension, however *.txt files are also compatible. After selecting a sequence file you will see the Sequence window, presented below. Move mouse over the areas of that window for explanations.
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At this point you may modify your sequence, search for primers/probes or use any of the analyze options.