Search: Scoring System
In Oligo 7 we have introduced user-controlled scoring system. Each primer, probe or set has its own score. The more points the better your oligo is. However, with so many different applications and systems it is not possible to allocate fixed number of points to any single feature or parameter. While hybridization probes should be primarily judged by their melting temperatures, the PCR primers should not dimerize, especially on their 3'-ends, for example. Therefore, you will see a different score for exactly the same oligo, depending on what type of search is selected. You may also change the penalties an oligo receives. This could have a drastic impact on the search results, so caution is advised: do not change these values if you don't know exactly what you're doing.